Can anyone with expertise in slice culture optimisation & validation, please suggest to me how I can troubleshoot staining of DAPI and PI?

After taking 250micrometer thick rodent brain section (by vibratome in aCSF) and incubate them with 1mg/ml of PI for 20 mins and then 1microgram of DAPI for next 40 min ( with/without fixing the...

06 January 2017 157 1 View