Yasaman Moradi

5 Questions 2 Answers 0 Followers

Questions related from Yasaman Moradi

Is there a relationship between the concentration of tracer (labeled Ag) and the maximum measurable concentration of analyte in a competitive ELISA?

In order to find the optimum concentration of my tracer, I replaced it in a commercial kit instead of kit's tracer, in the range of 5-150 ng/ml, but I was surprised to see the OD was similar and...

11 November 2018 6,260 1 View

How can I calculate service life of one electrical device ?

i have designed electrical device and now I should inform service life of the device.but I dont know how.

12 December 2017 3,749 0 View

I would like to know how can I prolong the shelf life of a coated 96 wells plate in 2-8 c in by modifications in coating step.

What are all parameters of coating antibody on a plate which should be considers?

07 July 2017 8,911 1 View

Is there a functional and effective ribosme binding site for cloning two genes with one promoter in Pseudomonas aeruginosa?

I want to insert two genes in a vector and transform p.aer by this vector.I want to use RBS (ribosome bind site)in order to making the vector seq shorter.Is it possible?

03 March 2017 725 2 View

How the concentration of a labeled antigen should be optimized? Should this concentration be equal to the concentration of highest standard?

optimization of a competitive ELISA assay. I need to add labeled antigen and an unknown sample (or standard) simultaneously to the well which is pre-coated with capture antibody. labeled antigen...

02 February 2017 1,394 3 View