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Hi, I have done some gel-filtration chromatography and now that I want to plot my results, I am not sure if it should be UV absorbtion per time (min) or UV absorption per volume? Does anybody have...
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I noticed only one broad peak around 222 for the Far-UV spectra of my protein. It containes mostly alpha-helics but the 208 peak is missing. I have tested CD for both MBP-fusion protein and the...
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