Yara Saleh

3 Questions 4 Answers 0 Followers

Questions related from Yara Saleh

Lung lysis in RIPA?

I need to perform an ELISA to study cytokines in mice lung. I have like 20mg of lungs.. what is the volume of RIPA needed to lyse it? On the other hand, I would like to ask if you know if a lysis...

09 September 2017 9,480 1 View

Does anyone have a protocol to perform a Bronchoalveolar Lavage (BAL) on mice?

I was using a metallic probe to have access to the trachea and I inject 500µl of PBS (Phosphate-buffered saline). Using the Biorad Milliplex Kit to quantify the cytokines in BAL, I have almost...

04 April 2016 5,831 2 View

Have anyone used the kit "Mouse Quantikine ELISA Kit" from RnD systems?

I purchased these kits to test Il-6, and others cytokines in mice lung. This kit is usually used on serum, plasma and cell cultures supernatant but i want to use it on tissue homogenates (lung);...

01 January 1970 7,361 1 View