Yara Saleh

4 Questions 7 Answers 0 Followers

Questions related from Yara Saleh

Does anyone know the amount of PBMC needed in order to prepare a cell lysate with a good concentration of proteins?

I lysed 1 million PBMC in 100µl and 200 µl of a commercial cell lysis buffer (cell signaling) and then I measured the concentration of proteins with a Bradford assay. I compared two different...

09 February 2021 7,499 4 View

Lung lysis in RIPA?

I need to perform an ELISA to study cytokines in mice lung. I have like 20mg of lungs.. what is the volume of RIPA needed to lyse it? On the other hand, I would like to ask if you know if a lysis...

18 September 2017 5,361 1 View

Is it mandatory to add proteases cocktail inhibitor in order to quantify cytokines using a multiplex technology?

I am using the Biorad Bio-Plex Pro assay to quantify different cytokines in mice BAL (Bronchoalveolar Lavage), plasma, and lung samples. I have no idea if I should add proteases inhibitors to...

31 March 2016 5,614 5 View

What is the best way to collect blood from mice to obtain non hemolysed blood in order to study the circulating miRNAs?

I tried to collect blood by intracardiac puncture but all the samples where hemolysed, so I couldn't study the circulating miRNAs. On the other hand, what is the best temperature to stock the...

29 March 2016 8,239 3 View