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I would like to run some experiments about anti-cachexia drugs screening, using in vitro model of cachexi. But after I reviewed some articles I found that all researchers used in vivo animal...
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I am detecting concentration of IL-6 in U266 cell line by western blotting. The result showed two blots, how could I solve this problem?
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The results of western blotting always show some non-specific blots, I wonder why it could happen, how to define the non-specific blots and how I can explain these blots when analyzing WB results.
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