3 Questions 12 Answers 0 Followers
Questions related from Yang Liu
I used to get a very high yield of plasmids with Promega maxiprep and miniprep kits, but it started to fall to +100ng/ul or 50ng/ul, respectively, for maxiprep or miniprep. I suspect that the...
07 July 2014 5,417 4 View
When I was growing Caco-2 BBe cells in an ICC staining chamber, the cells tended to overlap with each other, quite different from the status as they grow in p100 plates. As the cells are not...
02 February 2013 2,745 10 View
I am doing induction with Tet-on system. However, both of the tetracycline and doxycycline solutions I used failed to act as an inducer. I don't know how old the solutions are, but I'm suspecting...
02 February 2013 2,179 19 View