Yajing Sun

3 Questions 8 Answers 0 Followers

Questions related from Yajing Sun

How to solve flow cytometry reproductive issue: lose the cell surface bio-marker signal in flow cytometry?

I treated the cancer cell to induce calreticulin(CRT) exposure on the cell surface. Before I could observe 20% of ecto-CRT(+), then it dropped to 5%, then it dropped to nothing recently. I...

03 March 2019 9,724 7 View

Why the bands of my SDS PAGE horizontally spread?

I'm using the 15% hand casting gel for a 9 KDa protein, later a 4%-20% hand casted gradient gel. This problem the sample band became a line, and cannot be recognized which is which. It happened on...

06 June 2016 9,052 12 View

Why my beta-actin is unqual on western blot? Does it relate to lysis step?

Hello, I'm struggling with the unequal beta-actin expression level on western blot. I did the BCA twice and got the similar result, I'm sure the loading is correct. The loading amount should be 25...

05 May 2016 6,040 7 View