Yaghma Masood

2 Questions 8 Answers 0 Followers

Questions related from Yaghma Masood

Is this qPCR analysis for fold difference correct?

I have used FFPE HNC samples in my study. I couldn't get FFPE non-cancerous samples. So, the calibrators were FF (fresh frozen) non-cancerous samples from different patients. Therefore, for...

17 February 2017 5,847 4 View

Why do I get melt curve same as that of product but no amplification curve for my qPCR run without a template

I am using Bioline (2X master mix) with 10uM primer concentration as in the protocol. Can anyone help?

29 March 2016 7,906 8 View