Ya-Ping Chen

4 Questions 3 Answers 0 Followers

Questions related from Ya-Ping Chen

What kind of Fc receptor block should I choose for monocyte-derived macrophages?

In the past, I used 2.5 ug of Human BD Fc Block (564220) in each flow tube containg 0.5-1 x 10^6 monocytes from peripheral blood. The experiment results were very good. However, I used M-CSF to...

27 January 2016 545 2 View

Does anyone know how to do the FACS compensation for surface antigen and pHrodo E.coli bioparticle?

I isolate the monocytes from the peripheral blood and then I want to analyze the different phagocytosis ability in the subpopulations of monocytes by using surface antigen stain and pHrodo E.coli...

24 December 2015 3,733 1 View

Is there a phagocytosis assay using flow cytometry?

I isolate monocytes and macrophages from tissues. I need to stain surface markers, viability dye and phagocytosis at the same tube. What kinds of phagocytosis kits and viability dyes can I choose...

28 October 2015 5,103 7 View

How do I prove whether the cells are monocytes after RosetteSep™ Human Monocyte Enrichment Cocktail without CD16 depletion?

I use RosetteSep™ Human Monocyte Enrichment Cocktail without CD16 depletion to isolate the monocyte cells from normal peripheral blood mononuclear cells. Initially I used CD45, CD3, CD19 and CD56...

09 April 2015 4,870 3 View