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Hi everyone, I recently faced the trouble with bacterial contamination in cell culture, and it is not just as simple as discard all current cells and buying a new stock as many people suggest....
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I have been performing the Extracellular Flow Cytometry Procedure for detecting the extracellular protein and the data showed some reduction of protein expression (My protein is transmembrane...
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I want to measure the uptake of Flagellin after treatment to the cells. Please could anyone having experience for this experiment give me some suggestion. I tried to detect by Intracellular...
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Recently, I found that my protein of interest (Protein A) interacts with other proteins (Protein B) to form the complex. In principle, protein A always exists as the dimer, so based on the...
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Could anyone give me some advice about the elution of protein from acrylamide gel. I have searched some protocol, but I am still confused about the elution buffer they used in those experiments,...
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I am trying to extract matrix metalloproteinase-2 (MMP-2) protein from the cell. At first, I just used the common protocol (by RIPA kit) for whole cell proteins, but it seemed to be a wrong...
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Could anyone give me some suggestions about the "45Ca2+ blot overlay assay "? I searched for the procedure but there are some points that I could not understand. The general protocol is...
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