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There are many debates around the best way of telling the sensitivity of cancer cells to inhibitory drugs. such as GI50 (the dose giving Half inhibition on cell growth compared to non treated...
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Many vectors expressing shRNA or microRNA bear the U6 promoter, but what is the exact transcription start site? I have heard people put an additional G before their small RNA insert, is this G the...
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When I over expressed an E3 ligase Cbl mutant in a 3T3 cell line, the cell morphology changed. I have repeated this several times. Is this related to transformation? The cells are selected for...
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We would like to screen an RNAi library for metastasis players in human samples but not sure which cell line or assay should be used.
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I intend to isolate all the cells grown in soft-agar for genomic analysis. is there any protocol for this?
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