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Hello everyone, I have a paper under review. For my project, I did cell culture for MCF-7 and gave inhibitors to some groups. Substrate of E1 was added. Then the estradiol of supernatant was...
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The medium I use for cell culture of MCF7 includes DMEM medium, 10%FBS, 1% Glutamine, 1% ABS and 1% Na Pyruvate. I give the subculture for each generation every 3 or 4 days, with the concentration...
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