Xiaojing Sui

4 Questions 29 Answers 0 Followers

Questions related from Xiaojing Sui

How do we define the soluble proteins and insoluble proteins in cells? Is there any standard? Can anyone suggest me the corresponding lysis buffer?

I am working on separating the soluble and insoluble proteins in cells. However, after the literature searching, I found there are many different lysis buffers, which means the corresponding...

01 January 2015 1,547 4 View

How do you extract proteins from hippocampus of mice for 2D electrophoresis?

I am conducting 2DE of hippocampal proteins. The protocol is as follows: 1. Sample preparation: hippocampus were solubilized in the lysis buffer (7M Urea, 2M Thiourea, 4% Chaps, 30mM Tris-cl, pH...

04 April 2013 3,888 22 View

Can anyone tell me the interpretation of the following protein names?

Proteins identified by MALDI-TOF tandem MS/MS after 2D-DIGE analysis. SEQUEST was used for database searching against the NCBI. The results were as follows: 1. “RecName: Full=Hemopexin; Flags:...

03 March 2013 7,310 6 View

What's wrong with my gel?

I ran a 2D-DIGE gel, with a pH 4-7 strip (NL, 24cm). The sample is transgenic mice serum, which has been removed from albumin and IgG(Albumin and IgG removal kits, GE), then ultrafiltered with...

12 December 2012 7,726 23 View