Willie Anderson Dos Santos Vieira

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Questions related from Willie Anderson Dos Santos Vieira

Is there any problem to mix CTAB and SDS buffers? And can I add PVP in both or just in CTAB?

Im my experience, when i can't grind well my samples (fungi grown in PD broth), the SDS buffer provides more dna in the final of extraction. But, sometimes my pellet have some color, and I think...

30 January 2015 7,661 2 View

Why did a smeared DNA band appear and a second band next to the well?

Hi! Is attached a picture of the agarose 1% gel of my DNA extraction (fungi, Colletotrichum). The bands are smeared and appeared a second band next to the well. I used CTAB 2% as extraction...

12 January 2015 5,060 7 View