William Mills III

3 Questions 6 Answers 0 Followers

Questions related from William Mills III

Does anyone have any recommendations for an actin or tubulin antibody to use for IHC in mouse brain tissue?

I unfortunately can't use alpha smooth muscle actin. Rabbit is the preferred species. Mouse is not ideal.

22 February 2018 8,175 3 View

Anyone have a snap freezing protocol involving 2-methylbutane or equivalent reagent and dry ice? What is your storage temp?How long before sectioning?

My current protocol involves 2-methylbutane and dry ice. I do everything I can to ensure all reagents are as cold as possible so that freezing time is below 1 minute. I store 2-methylbutane at...

16 February 2018 8,341 5 View

I am trying to immunostain ZO-1 in 50 micron, 4% PFA fixed mouse brain tissue and am having difficulty obtaining signal. Could someone offer help?

I have read papers highlighting ZO-1's sensitivity to overfixation, and where best results were obtained with thinner sections (10 to 20 microns). Going forward I would like to try this, but for...

08 September 2016 6,868 24 View