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After an expression plasmid transformation into E coli, which event will come first? Replication or expression?
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I've faced a weird problem. My target DNA methyltransferase was cloned into pTrcHisA expressionvector. The expression was carried by both IPTG and autoinduction system. After induction I've tried...
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I've faced a little problem. I used E. coli to express an archaeal protein that should be thermostable. When I used heat-treatment to remove the bulk of the endogenous E. coli protein for...
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