Willem Finn Borbein

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Questions related from Willem Finn Borbein

Is there a recommended cell permeable IP3R agonist?

I am looking for an IP3R agonist for intact cardiomyocytes. Compounds like adenophostin A, or Gq-agonists (phenylephrine, endothelin) are unfortunately out of the question. I would be very pleased...

12 April 2021 7,568 4 View

Why do I have GFP bleaching in the process of immunostaining?

I am working with AAV transduced cardiomyocytes expressing an ER standing GFP. When I fix the cardiomyocytes in 4%PFA and stain them with additional antibodies, the previously strong GFP signal is...

03 February 2021 867 4 View