Wenzel Chen

2 Questions 3 Answers 0 Followers

Questions related from Wenzel Chen

How long can I store PCR products (before and after DpnI treatment) at 4°C or -20°C?

Hi, I recently working on PCR-based site-directed mutagenesis, and I have some questions about the stability and storage of PCR products (before and after DpnI treatment). How long can I store...

23 October 2021 6,575 3 View

How could I prevent the smear and unexpected bands in gel after restriction enzyme digestion?

Hello, I performed a single digestion on my plasmids (~6.5kb) using BamHI. The total reaction volume was 10µl (containing 0.5µl(10U) BamHI) and the samples were incubated at 37° for 1hr. After...

25 August 2021 7,269 3 View