Wenxiu Ning

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Questions related from Wenxiu Ning

How to dissolve matrigel conpletely to release organoids?

Hey guys! I am wondering if you have a perfect way to dissolve matrigel completely? I was trying to release organoid and want to select aorganoids that were smaller than 40 um. I tried put the...

07 July 2023 3,280 2 View

Ligation vector with DNA fragment failed, and don't know why. Vector ~8.2kb; DNA fragment ~1kb. Can anyone help?

I want to ligate x-vector with one DNA fragment. The vector was digested using BspE1 and Bgl II, and the DNA fragment was digested using BspE1 and BamhI, I used TAKARA solution I to ligate them,...

02 September 2014 2,530 15 View

How can I discriminate between the siRNA knock down effect and unsatisfactory conditions of the cell itself?

I checked my siRNA knock down efficiency using WB, and my protein A was somewhat knocked down. However, when I did time lapse to see its knock down effects on the other protein B-GFP, the cell did...

30 January 2013 9,594 8 View