Wenxi Xu

5 Questions 10 Answers 0 Followers

Questions related from Wenxi Xu

In the mtb-infected mouse model, should I do the perfusion if I want to harvest the lung and monitor the organ CFU?

Normally, if we need to isolate the lymphocytes from the lungs, perfusion through the heart is necessary, by which the blood lymphocyte contamination will be excluded and less red blood cells...

05 November 2016 9,298 1 View

Have anyone tried the antibiotic combination of 7H11 selectable agar on plating mice organ homogenate?

Hi guys, I am here again with mycobacteria question. for plating organ homogenate from SPF mouse, we have to add antibiotic combination to the 7H11 agar. the combination I used for BalB/c mice...

19 October 2016 1,589 6 View

Is there anything in blood co-relative to the bacterial burden in MTB infected mice?

Hi guys, I am working with the mtb-infected mouse model. and i am thinking about whether we can monitor the bacterial burden with mouse blood. the current way we do for the bacterial burden...

06 October 2016 4,933 3 View

What is the sequence of wild type GP33 in LCMV?

I have a question about the epitope GP33 of LCMV. In some paper, it is indicated that the wild type GP33-41 is KAVYNFATM, yet in others, the wild type GP33-41 turned to be KAVYNFATC. Which one is...

27 September 2016 9,421 3 View

How should I do the colony PCR on Mycobacterium?

I found a protocol for colony PCR in the book 'Mycobacteria protocols': "Colony PCR is a quick method to check for DCOs. A loopful of cells is resuspended in 400 uL sterile distilled water and...

12 April 2016 4,746 6 View