Weikan Wang

3 Questions 1 Answers 0 Followers

Questions related from Weikan Wang

Do I need to stimulate T cell with PMA and Ionomycin before single cell RNA seq for cytokine mRNA expression?

I would like to isolate T cells from mice with inflammatory disease and perform single cell RNA sequencing and detect the cytokine mRNA profiles. do I need to stimulate T cells with PMA and...

31 October 2020 6,113 2 View

What is the time point to sacrifice mice in EAE experiment?

I am just starting my project on experimental autoimmune encephalomyelitis (EAE). as the EAE clinical score is changing with time, what is the time point That I should sacrifice my mice for sample...

07 June 2019 3,552 4 View

How can I get neural stem cells for flow cytometry?

I want to get neural stem cells for flow cytometry. Can I use some percentage of percoll to get them? if possible, what percentage am I supposed to use?

20 November 2016 501 1 View