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I have a plasmid encoding my gene of interest which has GC rich DNA sequence , which fully sequenced only in presence of 10% DMSO. This plasmid was transfected in HEK293 cells . After selection...
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Hi 1- I did transient transfection for hek293 cells by 6 kb plasmid after 48 h the cells expressed my target protein at approximately 60%, I used lipofectamine 2000 in a DNA: LIPO ratio 1:4 then...
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