Wedad Sarawi

3 Questions 2 Answers 0 Followers

Questions related from Wedad Sarawi

What might be the reasons of truncation of the stably over-expressed proteins?

I have a plasmid encoding my gene of interest which has GC rich DNA sequence , which fully sequenced only in presence of 10% DMSO. This plasmid was transfected in HEK293 cells . After selection...

08 November 2018 4,379 2 View

Generating a stable transfection?

Hi 1- I did transient transfection for hek293 cells by 6 kb plasmid after 48 h the cells expressed my target protein at approximately 60%, I used lipofectamine 2000 in a DNA: LIPO ratio 1:4 then...

10 January 2017 8,429 5 View

What are the possible reasons for the failed sequence after the site directed mutagnesis with one primer ?

hello I am working on subcloning to insert my target protein to new vector, after the restriction, ligation,and transformation I did sequencing for the DNA by the vector primers VP1.5 nad XL39 and...

10 December 2016 5,905 4 View