Waseem Akram Malla

4 Questions 23 Answers 0 Followers

Questions related from Waseem Akram Malla

What is the final volume of "Lipofectamine-2000+gene of interest+Opti-MEM" mixture per well in which you incubate your cells in a 6-well plate?

I'm trying to ascertain the volume of the transfection mixture in which the cells are incubated for transfection to occur. I consulted a few documents/protocols (links below) and what I understand...

11 November 2018 6,756 4 View

How good is MinION for RNA-Seq/transcriptome studies?

I want to know how reliable, cost efficient and accurate is the data generated in MinION for studies involving transcriptomics. If you have used it, shed some light on the error rate, estimated...

10 October 2018 9,241 5 View

When is a cell in a culture deemed to have passed from one passage to the next?

In an animal cell culture, when do we consider a cell to have passed from passage Pn to passage Pn+1? Consider a scenario where I've a flask containing P20 (twentieth passage) of a cell line. I...

09 September 2018 7,218 6 View

Does anyone know what could this dark sticky material growing in my 4T1 cell culture flasks be?

I'm working with 4T1 cell culture. I have tried to establish the culture a few times and every time this dark sticky material sprouts in the flasks which increases with the passage of time, till...

05 May 2018 5,246 6 View