4 Questions 19 Answers 0 Followers
Questions related from Vu Hong Nguyen
Hello everyone, Currently I am working on synthesizing a single-stranded (ss) cDNA and I need to extract a specific band from gel (low melting point gel, SeaPlaque GTG agarose). I tried to follow...
03 March 2018 7,118 5 View
I am doing single cell isolation from tumor mass (using collagenase D & DNAse I) for FACS analysis. I also have 1 step for MACS cell separation after collecting single cell suspension....
03 March 2015 5,834 7 View
I am wondering how much we can see the correlation between FACS data and tissue staining? I am counting immune cells in a tumor (xenograft in mouse) using FACS with single cell isolation and also...
09 September 2013 3,896 2 View
I am trying to stain CD68 and CD11b on tumor tissue (mouse). I have collected mouse tumor tissue, fixed it in 4% Paraformaldehyde, dipped it in 30% sucrose and cryo in OCT -80oC. The tissues...
08 August 2013 4,749 11 View
