Vivian Montero-Alejo

4 Questions 10 Answers 0 Followers

Questions related from Vivian Montero-Alejo

Can someone help us and send an aliquot of E. coli ML-35 (ATCC 43827) lyophilized to our institute postal address?

I am preparing the inner membrane permeabilization assay and my last aliquot of E. coli ML-35 is not viable. I don´t have right now any other source to get the cells. Please, I know it is not...

10 October 2014 3,647 1 View

Can someone help me to decide which is the best procedure to obtain the monomer form without interfering with the antimicrobial test?

I have dimeric and oligomeric forms of a 4,26 KDa peptide, it has positive net charge and is hydrophobic. I need to test the monomer in the antimicrobial assay.

09 September 2013 1,334 3 View

I'm losing peptides with the method of solid-phase extraction eluting with 80% ACN: H2O 0.1% TFA. How is this possible?

It is a cationic peptide of 40 aa. I used solid phase extraction as a concentration method, after a cation exchange that yields more than 97% purity, determined by RP-HPLC.

03 March 2013 9,975 11 View

How can I quickly know by modeling, if a peptide has possibilities for dimerization? Is there a software (open source) for such prediction?

I have a putative defensin like peptide (highly hydrophobic and charged) and I would like to know, by modeling, if it is possible that the dimer form likely exist, spontaneously. So, I need a way...

10 October 2012 6,960 0 View