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Questions related from Vivek Damodar Ranjan
I used a mixture of Pluronic F127 and F127DA to form a hydrogel with cells encapsulated inside by crosslinking with UV. It seems that the cells show very poor attachment to the hydrogel, also less...
22 April 2019 2,795 0 View
We are culturing cells inside a 3D PLGA electrospun scaffold. Currently, we are using Qiazol to isolate the RNA, and we found that the Qiazol dissolves the PLGA scaffold upon contact. And we also...
08 October 2018 1,461 4 View
When I coat the nanofibers using gold, prior to doing SEM, the fibers collapse and hence the pore size is reduced leading to incorrect results. I have used the lowest current available in the...
16 November 2016 4,971 5 View
I would like to know if plasma treatment is absolutely necessary for PLO and laminin coating of electrospun scaffolds made from PLLA and PLGA for a long term cell culture? Or if just dipping in...
14 November 2016 8,378 3 View
I am spinning PCL(solvent -chloroform/DMF) into an ethanol bath and then planning to freeze dry it to remove all traces of the toxic solvents. This is for a tissue engineering scaffold related...
22 September 2016 2,133 4 View