Vivek Damodar Ranjan

3 Questions 2 Answers 0 Followers

Questions related from Vivek Damodar Ranjan

Is Pluronic F127/Pluronic F127DA toxic to cells in crosslinked/uncrosslinked format?

I used a mixture of Pluronic F127 and F127DA to form a hydrogel with cells encapsulated inside by crosslinking with UV. It seems that the cells show very poor attachment to the hydrogel, also less...

22 April 2019 2,775 0 View

How do we isolate and extract RNA from cells cultured inside a 3D electrospun PLGA scaffold for qPCR analysis?

We are culturing cells inside a 3D PLGA electrospun scaffold. Currently, we are using Qiazol to isolate the RNA, and we found that the Qiazol dissolves the PLGA scaffold upon contact. And we also...

08 October 2018 1,421 4 View

Is plasma treatment necessary, prior to PLO/Laminin coating of electrospun scaffolds as compared to just dipping the scaffold in PLO/Laminin?

I would like to know if plasma treatment is absolutely necessary for PLO and laminin coating of electrospun scaffolds made from PLLA and PLGA for a long term cell culture? Or if just dipping in...

14 November 2016 8,356 3 View