Vishwanath Reddy Hothpet

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Questions related from Vishwanath Reddy Hothpet

How can we study the bacterial strain binding to paraffin embedded tissue sections?

I have paraffin embedded tissues. I need to study the binding of bacterial strains to tissue sections by immunoflourescence or immunohistochemistry. How can we study the bacterial strain binding...

11 November 2016 9,550 3 View

Is there an efficient method to purify soluble recombinant proteins from prokaryotic hosts by preventing formation of inclusion bodies or aggregation?

We have used pET 28(a) vector (E. coli) for cloning and tried to standardize induction protocol with so many trial and error methods regarding IPTG concentration variation (0.05 mM to 0.5 mM),...

01 January 2014 480 52 View