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I am isolating plasmid DNA using my own buffers and found the OD 260/280-1.93 and 260/230-2.19 the concentration is also as good as the commercial kit available in market. I have used 15ug/mL...
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When I am trying to change it from 1M Urea to 1XPBS it got precipitated. I am also using 300mM -500mM NaCl but it not helping to me.
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