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Questions related from Virlana M Shchuka
I am growing mouse embryonic stem cell-derived embryoid bodies (8 bodies per well of a 12-well palte) and was wondering if anyone knows how to prevent the bodies from clumping together within the...
06 March 2018 2,065 2 View
From time to time, when using the Neon transfection system, I do not see the side electrode "pulsing", which I would expect to see sometime between pressing the "start" command and receiving the...
13 August 2017 3,881 0 View
Hi everyone, I was wondering what the max amplicon size is for qPCR? All the past qPCRs I've done have successfully amplified target regions ranging from 80 bp to 400 bp. Now I have a target...
22 February 2017 7,050 4 View
Hello all, I am trying to generate a CRISPR-Cas9-mediated deletion in MCF-7 cells. To screen for the deletion in individual clonal cell populations, I need to sort individual GFP+ cells into wells...
12 December 2016 211 5 View
Hi all! A question from someone with very limited protein background: I want to generate a mutation in a particular amino acid-encoding codon via CRISPR HDR. I need to find out first whether this...
01 November 2016 6,716 5 View
