4 Questions 5 Answers 0 Followers
Questions related from Virlana M Shchuka
I am growing mouse embryonic stem cell-derived embryoid bodies (8 bodies per well of a 12-well palte) and was wondering if anyone knows how to prevent the bodies from clumping together within the...
06 March 2018 2,040 2 View
From time to time, when using the Neon transfection system, I do not see the side electrode "pulsing", which I would expect to see sometime between pressing the "start" command and receiving the...
13 August 2017 3,855 0 View
Hi everyone, I was wondering what the max amplicon size is for qPCR? All the past qPCRs I've done have successfully amplified target regions ranging from 80 bp to 400 bp. Now I have a target...
22 February 2017 7,017 4 View
Hello all, I am trying to generate a CRISPR-Cas9-mediated deletion in MCF-7 cells. To screen for the deletion in individual clonal cell populations, I need to sort individual GFP+ cells into wells...
12 December 2016 187 5 View
