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Questions related from Virginia Aragon
I am starting on a project doing live cell imaging using a widefield fluorescent microscope. I am transfecting a fluorescent protein and imaging with two channels at each time point. Do you know...
02 February 2015 1,168 4 View
I recently changed the Hg Lamp in our epifluorescent microscope and now for some reason the CFP and YFP channels do not work, they seem over-exposed even at exposure times as short as 10ms....
07 July 2014 3,661 6 View
I am developing a standard curve to determine the number of ligands per cell after a Cr3Cl coating protocol using flow cytometry. For this purpose, I am using beads that are precoated with an...
01 January 2014 9,226 2 View
I have been reading some papers regarding the dynamics of receptor-ligand binding for my project and have come across this term "dynamic affinity" quite often and I am wondering if it actually has...
10 October 2013 1,814 2 View
I am trying to transfect HPMEC using Lipofectamine 2000. I already tried transfecting for 6h using serum-free media (otimem) and the cell viability dramatically decreased, I reduced the...
09 September 2013 1,467 4 View
Does anyone know an accurate technique to quantify intracellular protein concentration besides ELISA? I need a reliable and highly quantitative method to measure protein concentration to gather...
06 June 2013 8,959 4 View
For the last couple of months I have been trying to establish an inducible HaCaT cell line using the TRex system from Invitrogen. I am following the standard protocol (first transfect with...
01 January 2013 3,350 16 View
Hey!!! Does anybody know whether MDA-MB-231 cells are sensitive to Olaparib? If not, does someone know which cell line is sensitive to Olaparib and what should be the concentration of Olaparib to...
02 February 2012 4,836 2 View
