Virendra V. Panpatil

2 Questions 9 Answers 0 Followers

Questions related from Virendra V. Panpatil

What should be the treatment duration of ZnSo4 for cell line study?

We would like to replete cells with ZnSO4 to see the effect on expression levels of Zn-transporters. So What can be best time duration for ZnSO4 treatment...like 2, 4, 6, 12, 24 or... hours?

28 August 2019 5,875 1 View

I performed a qPCR for one gene . The results shows 4 samples peak at different Tm in melt curve what could be the reason?

I performed a qPCR for one gene in THP1 cell line cDNA (32 samples and 6 standards). The results shows 3 samples and one standard peak at different Tm in melt curve. What could be the reason?

28 December 2017 5,559 3 View