Vini Gautam

4 Questions 18 Answers 0 Followers

Questions related from Vini Gautam

What are the reasons for dead cells in primary hippocampal culture?

I have been trying to grow primary culture from isolated hippocampi from rat pups. Sometimes the cells survive while most other times they strangely land up dead like debris after 3-4 days. I have...

10 October 2015 1,945 12 View

What might cause cells to "burst" after taking them out of the incubator?

I have been growing neuroblastoma B50 cells on glass coverslips and the cells appear healthy when I see them under the normal microscope. For electrophysiology, I take the coverslip out and place...

02 February 2015 1,396 4 View

Is 400 mOsmol/kg too high for cell culture?

I have been doing cell culture with B50 cell lines recently and the cells have not been showing the expected neuronal morphology, even after reducing the serum concentration. I just happened to do...

01 January 2015 6,421 19 View

What is the best incubation temperature for neuronal cells?

I was wondering what effect does the variation in incubation temperature have on the growing cells, specifically neuronal cells? I read that mammalian cells should be incubated at 37 deg, but the...

09 September 2014 5,880 7 View