Vineeth Kumar Murali

5 Questions 5 Answers 0 Followers

Questions related from Vineeth Kumar Murali

Can you use RIPA buffer to extract membrane proteins from cells? if not whats the best way to prep lysates to detect membrane protein?

I have used RIPA buffer following the protocol listed on a Nature paper. But I have not been able to detect the protein of interest. Have tried three different sources for the antibody and also...

09 December 2022 5,296 2 View

Whats the optimum dose of TNFa neutralizing Ab for an invivo study?

Has anyone used any of the mouse TNFa neutralizing antibody in vivo? Whats the ideal dose that i could possibly use?

10 September 2019 5,914 1 View

Has anyone observed a fluctuation in blast count on mouse blood every week?

I am working on PDXs and to monitor tumor progress I bleed my mice every 7 days. I have noticed a wild fluctuation in blast percentage from week to week. Is that normal or should I tweak my...

15 September 2017 7,533 0 View

Is there a way to quantify Alkaline Phosphatase on cell lines?

I plated my cells on a 96 well plate and stimulated them with BMPs. After 5 days I aim to detect the Alkaline Phosphatase by using Sigma's Leukocyte Alkaline Phosphatase Assay Kit. I get a...

17 August 2015 2,373 3 View

Is there any difference in the way the apoptosis pathway is activated in B and T lymphocytes?

I am trying to look at what apoptotic genes are activated after treating the B-cell leukemic cell lines with Dexamethasone. I have gone over some literature on the genes that are activated in T-...

12 March 2014 2,526 2 View