Vincent J. Coppola

5 Questions 2 Answers 0 Followers

Questions related from Vincent J. Coppola

Can cresyl violet be used as a counterstain replacement for hematoxylin?

I am staining for amyloid plaques using Sigma-Aldrich's congo red kit. The first step is counterstaining with Mayer’s Hematoxylin Solution or Hematoxylin Solution, Gill No. 3. Can cresyl violet...

04 November 2019 8,713 1 View

Large amount of myelin stained after IHC for Bax (Ser184)?

Can anyone explain to me why I got a large amount of myelin staining when I did IHC for Bax (Ser184) using the ABC method and DAB? I know Bax is associated with apoptotic neurons, which is what I...

22 May 2016 6,391 2 View

Does anyone know if there exists a matrix for pigeon brain sectioning?

There seem to be matrices for sectioning rat, mouse, monkey, pig, dog, and cat brains. Does anyone know if a pigeon brain matrix even exists? Has anyone rigged up their own matrix for sectioning...

08 July 2015 6,295 0 View

Best way to visualize diagonal band in avian brain?

I would like to apply a stain that will really make the diagonal band in the pigeon brain "pop-out". I can identify it in cresyl violet-stained sections, but sometimes it is quite difficult to...

21 April 2015 5,175 3 View

Can anyone provide some insight with the following BrdU concerns?

I have recently begun working with BrdU to label proliferating cells in the VZ of pigeons (thus, this is a 24 hr survival) -- the quality of the IHC has been less than impressive, but that's...

10 March 2015 2,513 1 View