3 Questions 6 Answers 0 Followers
Questions related from Vikashini Balasubramanian
Hello All, I have a cpDNA sequence (Both forward and reverse sequence) of some barcode genes. Can anyone guide me further for gene annotation (Step by step procedure) and submission in NCBI. Is...
03 March 2017 9,783 2 View
In gradient PCR i get amplicons at all temperatures from 50-60 degrees. Is this correct? Why this happens so? Which temperature should i finally fix as annealing temperature for future works?
26 September 2016 4,122 5 View
Can anyone suggest how to decide the annealing temperature from the Tm value of primers?
08 September 2016 7,623 4 View
