Vidyalatha Kolli

6 Questions 11 Answers 0 Followers

Questions related from Vidyalatha Kolli

Getting lot of noise in the CD spectra at 190 to 210nm?

i was trying to take far UV spectra of a protein sample (buffer: 10 mM NaPi, 100mM NaCl, 2mM DTT). Till 210 nm there is no noise in the spectra but in the range 190 to 210nm there is lots of noice...

11 November 2018 1,490 12 View

Molecular simulation dynamic study of protein?

How to calculate the number of Na and Cl inos to be added to maintain 100mM NaCl concentration in the system, for molecular dynamics simulation study of a protein?

11 November 2018 2,507 0 View

Can anyone suggest what is the reason behind 3T3 cells growing in clumps ?

I have seeded NIH-3T3 cells in T-25 flask. After 24 h, cells start to form clumps and continued to grow in the same manner.(Cells were grown in high glucose DMEM, 10%FBS, 1% antibiotic)

08 August 2018 4,681 4 View

Getting two absorbance peaks for purified protein sample, one at 277 nm and another at 230 nm by UV spectroscopy?

hi, I am purifying the FHF2 protein by, Ni-NTA column chromatography followed by heparin column chromatography. After purification I have loaded the purified sample in SDS page gel, there is a...

01 January 2018 3,237 7 View

Does methylation of DNA affect the binding of ETBR??

Does methylation of DNA affect the binding of ETBR?? Single stranded plasmid synthesis can be possible?

09 September 2014 5,930 5 View

Can anyone help me troubleshoot a problem I've been having with agarose gel electrophoresis?

Agarose gel electrophoresis, I have ran (2ul sample+1ul 6X gel loading dye+ 3 ul sterile water) plasmid (6kb) sample in 1 % agarose (Low EEO grade) gel with Zip Ruler Express DNA Kb ladder (...

09 September 2014 8,997 5 View