Vidya Nandakumar

3 Questions 2 Answers 0 Followers

Questions related from Vidya Nandakumar

How can we analyze the Sanger sequencing result for detecting indels or substitutions? Is pair wise alignment enough for finding out indels?

How can we analyze the Sanger sequencing result for detecting indels or substitutions? Is pair-wise alignment enough for finding out indels or is any particular software available for finding the...

29 April 2023 3,502 3 View

PAM sequence showed one substitution but no mutations in guide RNA. Is it edited or not?

I got the sequencing result for CRISPR knock-out experiment, but guide RNA did not show any mutations but the PAM sequence showed a single substitution. However, the sequences flanking the guide...

05 January 2023 5,169 1 View

Should I use forward or reverse strand for finding mutation in a gene via CRISPR experiments?

I am trying to knock down a gene in soybean (Indian cultivar). After sequencing the genomic DNA from leaves, I am getting 97% similarity between forward seq and ref seq from NCBI whereas only 82%...

03 May 2022 5,576 1 View