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Questions related from Victor Tudorica
The protein in question is the Drosophila Pxd Peroxidase and it has been expressed in Pichia Pastoris system. I would like to know if viable expression in an E. coli expression system is possible.
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I have a set of long targets to amplify( vary between 3 kb and 15 kb) and not sure what enzyme/ enzyme mixtures work best. I have read about the Herculase II Fusion DNA polymerase and the Elongase...
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