Victor Llombart

2 Questions 6 Answers 0 Followers

Questions related from Victor Llombart

Can anyone suggest us a buffer to detach exosomes from magnetic beads?

We are trying to isolate CD63+ exosomes in order to characterize them by electronic microscopy, Nanosight and ultimately by proteomics/transcriptomics. To isolate them we use the “Total Exosome...

21 November 2016 1,178 16 View

What is the best strategy to validate SILAC results by WB?

In our SILAC experiment we analyzed supernatants from cell cultures. It seems that treated cells secrete more protein than control cells (more or less 3 fold, as checked in many other cultures we...

12 December 2013 5,504 1 View