Victor Llombart

3 Questions 8 Answers 0 Followers

Questions related from Victor Llombart

Can anyone suggest us a buffer to detach exosomes from magnetic beads?

We are trying to isolate CD63+ exosomes in order to characterize them by electronic microscopy, Nanosight and ultimately by proteomics/transcriptomics. To isolate them we use the “Total Exosome...

21 November 2016 1,236 16 View

Can anyone help with laser microdissection of brain vessels?

We are trying to isolate brain vessels from human necropsies through laser microdissection and we're having some troubles with the staining with leptin. Could anyone suggest an alternative...

17 January 2014 8,986 3 View

What is the best strategy to validate SILAC results by WB?

In our SILAC experiment we analyzed supernatants from cell cultures. It seems that treated cells secrete more protein than control cells (more or less 3 fold, as checked in many other cultures we...

12 December 2013 5,541 1 View