Vicky Gent

3 Questions 6 Answers 0 Followers

Questions related from Vicky Gent

What is the best statistic to use to compare groups of proliferating PBMCs?

Hello everyone. I collected PBMCS from 9 animals (3 groups) at 6 timepoints. Then I did some PBMC cultures (72hours) with CFSE with certain a certain Antigen (with CONA as a positive control and...

22 November 2017 7,082 4 View

Is it possible to store CFSE stained PBMCs for 48hours in 4 degrees without fixing?

Hi everyone. I would like to isolate some PBMCs which I stained with CFSE and cultured for 72 hours, however I won't be able to analyse them with flow immediately, possible after 48hours, is it...

31 July 2017 4,723 11 View

What is the optimum number of events to run on the flowcytometer for a lymphocyte proliferation assay

Hello everyone. I'm currently doing some lymphocyte proliferation assays with CFSE. I cultured 1X10^6 cells per well and stained with 5mM CFSE and stimulated them with certain Antigens including...

18 July 2017 8,529 7 View