Vera Abramova

3 Questions 7 Answers 0 Followers

Questions related from Vera Abramova

Can Monensin replace Brefeldin in IFNg analysis?

Can Monensin be used INSTEAD of Brefeldin upon intracellular analysis of IFNg? (mouse splenocytes, antigen specific stimulation)

30 June 2017 1,453 1 View

T cell proliferation assay -whole splenocytes or T cell isolation is obligatory?

I'm looking for T cell proliferation assay setup. Has it a rationale to use splenocytes from two congeneic mice 1) as responders (from immunized mice, cfse labeled) and 2) as APC from the second...

09 January 2017 4,114 5 View

Immunoglobulin sequence coverage by a set of primers-how to determine?

I have a set of forward primers (and a reverse but it s OK) to amplify region in murine Immunoglobulin cDNA. Forwards are for FR1 region of VH. With which (online) tools i can determine the...

07 December 2016 258 2 View