Venkatesh Kumaresan

4 Questions 19 Answers 0 Followers

Questions related from Venkatesh Kumaresan

Is it necessary to include PCR efficiency E value in relative quantification? Is it mandatory according to MIQE guidelines?

When we do relative quantification of test gene with a house keeping gene, is it necessary to include PCR efficiency? If yes, Could someone provide reference? Does MIQE guidelines made it...

10 October 2014 4,328 5 View

Should more identical sequences come together in a phylogenetic tree?

We have constructed a neighbor joining tree in MEGA with few eukaryotic protein sequences. According to BLAST result, our query shared 96% identity with Organism A and 93% identity with Organism...

04 April 2014 3,259 6 View

How to find immune related genes from a whole genome sequence?

How can I identify immune related genes from a whole genome sequence? I am planning to identify immune genes and find the expression of those genes. Any suggestions?

12 December 2013 3,744 2 View

Can someone advise on why is the restriction double digestion not working?

gel. Where as clear band was seen in the lane where uncut plasmid is loaded. Now I am planning to set

07 July 2013 2,213 9 View