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Questions related from Venkatesh Mandari
I am working on an enzyme production in submerged fermentation. I already have some data available from my previous batch. They have worked with 5 different variables (substrate concentrations) to...
30 October 2023 7,126 0 View
I have produced lipase using ssf using aspergillus niger mtcc 872. Now, I want to purify the lipase. During the ammonium sulphate precipitation, it is mentioned 0-80% or 10-80% saturation. what...
12 July 2021 2,157 3 View
I am trying to analyze lipase using the reverse phase-HPLC C18 column. In the literature, 0.1 % TFA was and 60% ACN was used. Can I use TCA in place of TFA? If any other methods are available to...
26 February 2021 2,900 3 View
I am working on protein (lipase enzyme) purification. After using the Sephadex G-25 fine column, which column (Q or DEAE sepharose column) should I use? What are the buffers should I select? Thank you
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I am analyzing the biodiesel using reverse-phase HPLC. Methyl Oleate and Methyl Palmitate are overlapping in a single peak. How can I quantify the individual components from the same peak?
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