Venkatesh Mandari

4 Questions 9 Answers 0 Followers

Questions related from Venkatesh Mandari

How to study the interaction effect of different variables and optimize the product?

I am working on an enzyme production in submerged fermentation. I already have some data available from my previous batch. They have worked with 5 different variables (substrate concentrations) to...

30 October 2023 7,038 0 View

Can I use TCA instead of TFA for protein analyis in RP-HPLC?

I am trying to analyze lipase using the reverse phase-HPLC C18 column. In the literature, 0.1 % TFA was and 60% ACN was used. Can I use TCA in place of TFA? If any other methods are available to...

26 February 2021 2,726 3 View

What is the difference between Q sepharose column and DEAE Sepharose column?

I am working on protein (lipase enzyme) purification. After using the Sephadex G-25 fine column, which column (Q or DEAE sepharose column) should I use? What are the buffers should I select? Thank you

17 February 2021 7,176 5 View

How to quantify the two components overlapping in HPLC?

I am analyzing the biodiesel using reverse-phase HPLC. Methyl Oleate and Methyl Palmitate are overlapping in a single peak. How can I quantify the individual components from the same peak?

15 November 2019 1,545 9 View