Venkatasubramanian Vidhyasagar

5 Questions 3 Answers 0 Followers

Questions related from Venkatasubramanian Vidhyasagar

Why are there two peaks in protein Q-TOF?

Hi All, I have purified a His-tagged recombinant human protein and performed Q-TOF for determining molecular mass. However, I get two peaks all time with mass difference of 131 Da (deconvoluted...

12 October 2015 8,333 5 View

Has anyone faced sequencing problem for pSpCas9(BB)-2A-Puro (PX459) V2.0?

Hi We have cloned our sgRNA into BbsI site of CRISPR vector pSpCas9(BB)-2A-Puro (PX459) V2.0. Digestion with Age I and BbsI showed most of clones have insert (no 1 kb band compared with empty...

30 July 2015 6,613 3 View

Are there any drugs for mediating hypermetylation?

Hi, I need to decrease or stop a gene expression caused because of being hypomethylated. Is there any possibility to shut off the gene by hypermethylation? Although not obvious to be specific in...

27 February 2015 7,251 3 View

How do I increase the expression of proteins?

Hi, I have cloned a protein in pET 28a and expressed in Rosetta 2 cells. I could detect the protein with anti His antibody and also protein specific antibody in western. However on purification, I...

09 October 2014 4,586 4 View

Does anyone have any suggestions for solving some problems with the expression of a protein in Rosetta 2?

I have cloned a mammalian protein in pGEX vector (GST tag) and transformed it in Rosetta 2 cells. I could get good expression earlier and used 1 ml of beads (washed and suspended in PBST) however...

20 May 2014 3,284 4 View