Venkatakrishna LM

4 Questions 7 Answers 0 Followers

Questions related from Venkatakrishna LM

Any suggestions for Buffer exchange from high denaturant agents to Ammonium Bicarbonate for LC-MS/MS analysis?

My whole lysate proteins were prepared in 8M Urea buffer +2M Thio-Urea+ 4% CHAPS. In preparation for LC-MS/MS analysis, the samples need to undergo desalting and be switched to Ammonium...

11 August 2023 7,155 2 View

Freezing Protocol for Caenorhabditis elegans?

Can anyone suggest me a best protocol for freezing worms using glycerol storage? I've also tried the protocol from wormbase and faced more trouble shoot in reviving the worms though it has been...

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I'm working with Liver cancer cell in my lab. Can anyone suggest me a protocol for Sonicating the cells without using any "Lysis buffer" ?

Need a protocol for sonicating the Hep3B cells using Ultra-sonication Instrument.

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Anyone know the native neuropeptide proteins isolation from C. elegans for LC-MS/MS?

I am in need of a protocol aimed at selectively isolating neuropeptides from a comprehensive protein lysate, either preceding or concomitant with LC-MS/MS analysis. I am inquiring whether...

01 January 1970 5,449 5 View