Venkatakrishna LM

5 Questions 7 Answers 0 Followers

Questions related from Venkatakrishna LM

How to knock-in genes with multi-epitope tagging in C. elegans using CRISPR/Cas9 gene editing technique?

I am interested in inserting a gRNA into the genome of C. elegans and tagging multiple epitopes at both the prodomain and mature domain regions of the target signal sequence to observe the...

09 October 2024 7,307 0 View

Any suggestions for Buffer exchange from high denaturant agents to Ammonium Bicarbonate for LC-MS/MS analysis?

My whole lysate proteins were prepared in 8M Urea buffer +2M Thio-Urea+ 4% CHAPS. In preparation for LC-MS/MS analysis, the samples need to undergo desalting and be switched to Ammonium...

11 August 2023 7,212 2 View

Freezing Protocol for Caenorhabditis elegans?

Can anyone suggest me a best protocol for freezing worms using glycerol storage? I've also tried the protocol from wormbase and faced more trouble shoot in reviving the worms though it has been...

22 September 2020 7,214 3 View

I'm working with Liver cancer cell in my lab. Can anyone suggest me a protocol for Sonicating the cells without using any "Lysis buffer" ?

Need a protocol for sonicating the Hep3B cells using Ultra-sonication Instrument.

15 December 2018 3,214 4 View

Anyone know the native neuropeptide proteins isolation from C. elegans for LC-MS/MS?

I am in need of a protocol aimed at selectively isolating neuropeptides from a comprehensive protein lysate, either preceding or concomitant with LC-MS/MS analysis. I am inquiring whether...

01 January 1970 5,509 5 View