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Questions related from Vanessa Smilansky
I am looking to validate an ATAC-seq protocol for which I need isolated nuclei. I have found a number of protocols and kits for isolation of nuclei from cells, but I would prefer pre-isolated...
21 February 2023 2,982 2 View
Some CRISPR-based diagnostic assays combine RPA with CRISPR/Cas to detect nucleic acids. This apparently gives the assay an advantage by providing two rounds of specificity: primer-specific...
12 August 2021 5,260 1 View
From my understanding, microsatellite repeats should be consistent (e.g. di-, tri-, tetra-); therefore, allele sizes should be consistently separated by 2, 3, or 4 nucleotides. I am working with a...
05 April 2021 181 5 View
Hello, Excluding contamination and probe degradation, can anyone suggest a cause of late amplification in the NTC of TaqMan qPCR ? I considered the possibility of primer dimers, but since they...
22 March 2020 9,052 3 View
I'd like to measure inbreeding in a puma population over the course of seven years. The number of individuals representing each year is highly variable (~30 to ~150). Should I compare a subset...
13 July 2015 2,269 3 View
