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Some CRISPR-based diagnostic assays combine RPA with CRISPR/Cas to detect nucleic acids. This apparently gives the assay an advantage by providing two rounds of specificity: primer-specific...
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Hello, Excluding contamination and probe degradation, can anyone suggest a cause of late amplification in the NTC of TaqMan qPCR ? I considered the possibility of primer dimers, but since they...
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I'd like to measure inbreeding in a puma population over the course of seven years. The number of individuals representing each year is highly variable (~30 to ~150). Should I compare a subset...
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