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Questions related from Valerie J Carabetta
Our institute is in search of a new Phosphoimager. We currently have a Typhoon that no longer works. Can anyone give me a recommendation on what works well for them, or is the newest technology...
08 August 2014 9,996 4 View
I would like to do a native gel on a small (9 kDa) basic (pI-9.5) protein, and we will be looking to resolve different isoforms that have different PTM's. These PTM's will alter the pI...
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We have purified our protein of interest, and have shown that FAD is bound, by UV-VIS spectroscopy. We do not understand the role of the FAD binding. I would like to make mutations that disrupt...
09 September 2013 8,355 6 View
I am purifying a GST-tagged protein, and cleaving the tag with TEV protease. My buffer contains 20 mM HEPES, pH 7.5, 300 mM NaCl, 5 mM DTT. There is 75 mM reduced glutathione around from...
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