Valentina Faoro

3 Questions 3 Answers 0 Followers

Questions related from Valentina Faoro

I can't transform the recombinant pFB-Lic-Bse into DH10BAC. Do you have any suggestions?

Hi, I'm trying to transform E. coli DH10Bac cells with a pFB-Lic-Bse vector containing my gene of interest. 48 h after transformation, I get some white colony on LB-Km-Tet-Gent-Xgal-IPTG, but when...

10 October 2014 6,612 6 View

Can anyone suggest a protocol for the lentiviral transfection in neurospheres?

I'm looking for a protocol for lentiviral transfection of siRNA in neurospheres cell culture. Can anyone suggest a protocol?

11 November 2013 8,717 0 View

Are there any methods to avoid tissue section coming off from the slides in neurosphere cryosection?

I am working with neurospheres cryosection, fixed and embedded in OCT. When I try to stain the tissue sections they come off from the slides. I have used polarized slides, but they couldn't hol...

08 August 2012 3,424 3 View