Valentina Francia

5 Questions 7 Answers 0 Followers

Questions related from Valentina Francia

How to label proteins in an unspecific way?

Dear RG community, I need your help! I have a mixture of proteins attached to a surface. Some proteins are available for binding, others are not, because surrounded by other proteins. I need to...

06 May 2021 8,685 4 View

Can all iron oxide nanoparticles be considered superparamagnetic?

Dear researchers, Can all iron oxide nanoparticles be considered superparamagnetic? Or the "superparamagnetic" definition can be assigned only to certain types of iron oxide nanoparticles? If so,...

27 October 2020 784 5 View

What is the best chromatography (type, resin..) to separate blood lipoproteins among each other?

Dear all, I am trying to understand which is the "golden standard" method to separate lipoproteins from the blood (LDL, HDL, IDL, chylomicrons...). I found some methods online, and apparently...

11 August 2020 5,092 5 View

Which is your siRNA protocol for transfecting endothelial cells after monolayer formation?

Dear colleagues, I was wondering if someone have ever done such an experiment. I have successfully form an intact and compact cell monolayer with HUVEC cells and now I want to apply the protocol...

17 June 2015 4,000 4 View

How to deplete serum or plasma from a specific apolipoprotein?

Dear all, I need to deplete my serum/plasma from apolipoprotein E (apoE) and I have to be sure that my serum can still be used (sterile, in possible, and not altered). I also need to be sure that...

01 January 1970 3,300 1 View